LJK UN SD 2015 PDF

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Ljk Un Sd 2015 Pdf

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However, the correlation coefficients of the peak area relationships for the T 0: Similar findings have been reported by McCarthy et al. It is anticipated that with the optimization of the field unit, sample conservation similar to that of the lab tests can be achieved and no diversity will be lost.

In conclusion, BioDry is effective at preserving the key biomolecules DNA, RNAs, and proteins in single aquatic microbial samples with the integrity necessary for molecular analysis. This includes identification of not only the resident community, but also the active individuals via functional gene expression and catalytic proteins, collectively linking metabolic and biogeochemical activity.

Furthermore, preserved samples can be stored at room temperature for extended periods up to 30 d , indicating that biodegradative enzymatic activities e. This finding is in contrast with preservation of macromolecules using the most widely used liquid preservative, RNALater [ 4 , 7 , 9 ].

Likewise, it has still been recommended that flash freezing be employed whenever possible when preserving RNA molecules [ 17 ]. Conversely, once the portable system is optimized, BioDry will require only rechargeable batteries and desiccant. Finally, our findings indicate that, with optimization, a portable field version of the BioDry method will attain the level of preservation observed when using the laboratory device.

This could then be adapted for autonomous platforms to improve spatial and temporal resolution of biomass sampling and significantly improve our understanding of the factors controlling microbial populations in the ocean and almost any other environment.

Aquatic biomass can be collected using either a swinnex A or a syringe filter B while using either a syringe A or portable compressor B. A centrifuge tube is cut off at the bottom and a section of Tygon tubing is attached A. The newly filtered sample is placed inside the tube B , and then connected to another cut-off tube via a short length of tube C. This can then be fixed at either end to the in-lab apparatus see S1 Fig , or to the desiccation syringe of the portable apparatus D.

After desiccation is complete the caps are closed at either end for storage C. The portable apparatus consisted of a Ryobi compressor designed to filter and preserve aquatic samples. Plumbing was adapted to be successively diverted to first a sample syringe to load the sample onto a filter, then to a desiccant syringe to preserve the sample.

Panel A —Procedural control tests: Showing total nucleic acid extracts from filters that were either immediately frozen frozen controls , or subjected to the BioDry procedure, then flash frozen procedural control. Panel B —Sample integrity and procedural contamination tests: Results shown for both types of QC tests are representative of several such tests. The black arrows indicate the gain of a significant peak in multiple replicates within a treatment.

The red arrows indicate the loss of a significant peak in multiple replicates within a treatment. The authors wish to thank the Rutgers Office of Technology Commercialization and Robert Netter for the assistance in obtaining the provisional patent U. Provisional Application no. Environmental Proteomics N.

Kementerian Pendidikan Dan Kebudayaan: Lembar Jawaban Ujian

B, Inc. National Center for Biotechnology Information , U.

PLoS One. Published online Dec Steven J. Tuorto , 1 Chris M. Brown , 2 Kay D. Bidle , 1 Lora R. McGuinness , 1 and Lee J. Chris M. Brown 2 Environmental Proteomics N. Kay D.

Lora R. Lee J.

Newton CM Gomes, Editor. Author information Article notes Copyright and License information Disclaimer.

Inc, Sackville, New Brunswick, Canada,. Received Dec 19; Accepted Nov This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

AC powered vacuum pump adapted to BioDry filtered aquatic biomass samples. S2 Fig: Image of sample being collected onto a filter. S3 Fig: Pre-prepared filter holders.

S4 Fig: Image and schematic of portable BioDry apparatus.

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S6 Fig: Agarose gels from BioDry quality control tests. S2 Table: Closest match species taxonomic ID for sequences from the seawater samples— pyrosequence species identification closest match, percent similarity and relative percent contribution of organisms detected in the T 0 control and BioDried T 15 and T 30 samples.

Introduction Discerning the dynamic processes and interactions of natural microbial communities is key to understanding global biogeochemical cycles. In-Lab BioDry Apparatus and Sampling Procedure BioDrying was performed using a modified 50 ml centrifuge tube containing Drierite, an in-line air flow meter, and an AC powered vacuum pump, all of which were adapted to recirculate desiccated air over sample filters S1 Fig.

BioDry Method Verification Seawater from an ocean-reef aquarium was collected and incubated with 0. BioDry Field Verification The BioDry method was tested in the field using the portable apparatus S4 Fig on aquatic samples from both a saline and freshwater environment.

Results BioDry Method Verification In several sets of distinct preliminary tests, both the efficacy of the BioDry procedure on yield and integrity of biomass samples, as well as susceptibility of samples to contamination from the desiccation process were assessed via agarose gel and PCR amplification.

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